主要农作物 lncRNA 鉴定与分析研究进展

随着基因组和生物信息学技术的进步,特别是高通量测序技术的发展,人们发现了许多没有蛋白质编码潜力的转录单位。其中,长链非编码RNA(long non-coding RNAs,lncRNAs)是一种长度大于200 nt的非编码RNA,有着独特的二级结构且不编码蛋白质,lncRNAs可以在核内发挥作用,也可以在细胞质中发挥作用。大量的证据表明,lncRNAs几乎是每个细胞过程的调节器,其重要性引起了越来越多的人关注。lncRNAs主要与mRNA、DNA、蛋白质和miRNA相互作用,从而以多种方式在表观遗传、转录、转录后、翻译和翻译后水平调控基因表达。lncRNAs介导的调控在动物中已经被大量研究,而植物中的lncRNAs研究才刚刚起步,近期许多新的研究结果表明,lncRNAs在植物逆境胁迫、生长发育及系统进化的过程中起了非常重要的作用。概述了lncRNAs的来源、分类及lncRNAs在植物发育中的调控机制,列举了可用于植物lncRNAs分析的主要数据库资源,并就近年来玉米、水稻、小麦等主要农作物lncRNAs研究情况进行了梳理和总结,为今后深入挖掘植物lncRNAs的作用机制及其在农作物中的应用提供一定的参考。     

数字化人体模型在车身逆向布置中的应用

以人体参数为依据建立的数字化人体模型能有效的描述人体的形态特性.本文在CATIA人机工程模块中,建立了人群文件,生成自定义的三维数字化人体模型。在此基础上建立了满足不同百分位需求的参数化人体模型。以某汽车为对象,根据SAE标准,综合考虑了踏板行程、汽车视野和上下肢舒适性等因素对驾驶员的影响,最终得到满足各种约束的驾驶员最佳舒适性H点区域。论文研究为针对不同地区人群特性对车型进行逆向开发和逆向布置提供了很好的参考。     

南亚热带李新品种‘华蜜大蜜李’

 ‘华蜜大蜜李’是从‘三华李’中选育出来的优良新品种, 果实近圆形, 平均单果质量51.5 g, 可溶性固形物含量9%～11% , 酸甜味浓, 肉质软溶, 丰产稳产, 是‘三华李’中的大果品种, 适合于南亚热带山区栽培。在广东6月中下旬果实红熟。     

Effects of tirofiban on no-reflow and activation of NF-κB after myocardial ischemia/reperfusion in rats

AIM: To investigate the effects of platelet glycoprotein Ⅱb/Ⅲa receptor inhibitor tirofiban on myocardial no-reflow and activation of NF-κB after acute ischemia/reperfusion in rats. METHODS: Male Wistar rats were randomized into sham operation group, control group and tirofiban treatment group. Control group and tirofiban group were subjected ischemia for 90 min by ligation of coronary artery after thoracotomy and subsequently reperfusion for 120 min to establish acute myocardial ischemia/reperfusion no-reflow models. Thioflavine S, Evans blue and triphenyltetra zolium chloride (TTC) staining were performed to evaluate the area of no-reflow (ANR), infracted area (IA) and risk area (RA) of the heart. Immunohistochemistry was used for semi-quantitative analysis of the expression of nuclear factor-κB p65 (NF-κB p65) protein in myocytes and arteriole. Activity of myeloperoxidase (MPO) and content of malondialdehyde (MDA) in risk area of the heart were detected by ultraviolet spectrophotometer. RESULTS: After 120 min for reperfusion, compared to sham group, the statistical differences of higher positive expression of NF-κB p65 in myocytes and arteriole, activity of MPO and content of MDA both in control and tirofiban group were observed. Compared to control group, lower positive expression of NF-κB p65 in myocyte and arteriole, activity of MPO and content of MDA in tirofiban group were found (P<0.05, P<0.01). A markedly reduced ANR and IA were observed in tirofiban group than those in control group (34.36%±6.04% vs 52.09%±6.89%, P<0.01; 80.41%±8.48% vs 90.13%±5.72%, P<0.05). CONCLUSION: After myocardial ischemia/reperfusion for 120 min, no-reflow phenomenon can be observed in rats. Tirofiban reduces the areas of anatomic no-reflow and infarction, inhibits the activation of NF-κB in myocyte and arteriole, and decreases the infiltration of neutrophils and release of oxygen free radicals.     

灵芝多糖提取物胶囊对小鼠免疫功能的影响

通过设3个浓度的药物组和空白对照组,分别进行脏器指数测定,ConA诱导的小鼠脾淋巴细胞转化试验,绵羊红细胞诱导小鼠DTH试验,小鼠血清溶血素测定,抗体生成细胞检测,小鼠腹腔巨噬细胞吞噬鸡红细胞试验,小鼠碳廓清试验,NK细胞活性测定试验。结果表明,灵芝多糖提取物胶囊能增加ConA诱导的小鼠脾淋巴细胞的增殖能力和小鼠左后足跖部厚度差24 h测量值,提高小鼠血清溶血素抗体积数和溶血空斑数。表明灵芝多糖提取物胶囊具有较强的免疫调节作用。     

Soluble expression of a CXCL10-loop3-EGF fusion protein and its anti-tumor activity

AIM: To evaluate the implication of CXCL10-loop3-EGF fusion protein for the activities of targeting tumor and anti-angiopoiesis. METHODS: RT-PCR was preformed to amplify CXCL10 coding sequence from PBMC activated by IFN-γ. CXCL10-loop3-EGF fusion gene, which was conducted by Over-Lap Extention PCR, was hinged up with plasmid pTG19-T, transfected to E. coli DH5α and processed positive colony selection. After ligated with plasmid pET32a(+), recombinant CXCL10-loop3-EGF fusion gene was then transfected to E. coli Origami B (DE3) and induced to express its coding fusion protein his-CXCL10-loop3-EGF. The recombinant fusion protein CXCL10-loop3 -EGF was purified by His-bind affinity chromatograph, enterokinase cleavage, ultrafiltration and dislysis. The transwell chemotatic test and HUVEC angiopoiesis inhibition test were performed to determine the anti-tumor responses and anti-angiopoiesis activity of CXCL10-loop3-EGF fusion protein. RESULTS: CXCL10-loop3-EGF fusion protein was successfully constructed and confirmed by SDS-PAGE analysis and Western blotting. Significant PBMC chematatic activity and HUVEC anti-angiopoiesis activity were observed. CONCLUSION: CXCL10-loop3-EGF fusion protein, which has perfect anti-tumor activity, is successfully constructed.     

小波神经网络在模拟电路故障诊断中的应用研究

对模拟电路提出了一种基于小波与神经网络辅助式结合的故障诊断方法．该方法用小波变换作为模拟电路故障信号的预处理器，大大减少神经网络的输入数目，简化神经网络结构和减少它的训练时间，提高辨识故障能力．在介绍该故障诊断方法的基本原理后。给出了小波函数及故障特征选择的方法．     

参虾池塘高效混养技术

7-9月刺参夏眠期间正是日本对虾快速生长的季节,在对虾池中人工造礁,第1年4月放入规格为80～120头/ kg的海参苗1740 kg,每年7月放入体长0.7～1.0 cm的日本对虾虾苗1.2×105尾,将刺参与日本对虾混养.在养殖过程中,刺参不投喂,只用卤虫、人工饲料、杂色蛤和四角蛤蜊投喂日本对虾,严格控制养殖池内的杂鱼虾,保持水质新鲜,可提高日本对虾和刺参的成活率,利用两者饵料和空间互补性,可显著提高虾池的利用率和经济效益.     

低剂量核辐射在文蛤生产中的应用研究

用60 Co C射线辐照文蛤幼贝, 研究其对文蛤生长及抗病性的影响。A 组预试验结果表明: 低剂量60 Co C射线辐照对促生长有效, 高剂量辐射抑制了文蛤生长。B组正式试验辐照剂量(低剂量) 3. 23 @ 10- 7 ~ 25. 80 @ 10- 7C / kg。低剂量辐射试验结果表明: 46 d后, 试验组平均壳高及平均体重均高于对照组, 分别增加15% ~ 21% 和16% ~ 26%; 276 d后, 试验组的平均壳高较对照组增加17% ~ 23%, 且试验组文蛤的抗病性远优于对照组。61 45@ 10- 7 ~ 19. 35 @ 10- 7 C /kg 低剂量为促进文蛤幼贝生长、提高抗病性的有效剂量范围。